Local Temperature Measurement and Control Using Functional Gel-tool Containing a Quantum Dot by Color Analysis of Fluorescence Spectrum

We developed a novel temperature measurement and control method that uses color analysis of fluorescence spectrum. Functional gel-tools encapsulating quantum dots (Q-dots) was used. These tools can be manipulated in a solution by optical tweezers. We used color analysis of the fluorescence for temperature measurement. YCrCb information (Y: brightness, Cr: red color difference, Cb: blue color difference) is used for temperature measurement. From calibrations, we confirmed Cr information is suitable for temperature measurement with high sensitivity and stability. Moreover, we also demonstrated heat control of local environment using focused laser by simultaneous heating and temperature monitoring using the gel-tool.